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Code Name Applications Availability Size Tested species Price Grade  
EB05500-B Goat Anti-Plakoglobin / Gamma-catenin, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05500-B 1
Biotinylated EB05500 (2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: JUP, junction plakoglobin, ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB, catenin (cadherin-associated protein), gamma 80kDa, desmoplakin III, desmoplakin-3, catenin (cadherin-associated protein), gamma (80kD), GAMMA-CATENIN
Official Symbol: JUP
Accession Number(s): NP_002221.1, NP_068831.1
Human GeneID(s): 3728
Non-Human GeneID(s): 16480 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Immunogen

Peptide with sequence C-GLRPPYPTADHMLA., from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in lysates of cell line A431 (calculated MW of 81.7kDa according to Human NP_002221.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human

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