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Code Name Applications Availability Size Tested species Price Grade  
EB08421 Goat Anti-Pleiotrophin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08421 1
EB08421 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08421 2
EB08421 (0.1µg/ml) staining of Mouse fetal Brain (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTN, pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), HARP, HBGF8, HBNF, NEGF1, heparin affin regulatory protein, heparin binding growth factor 8, heparin-binding growth-associated molecule, neurite growth-promoting factor 1, pleiotrophin, HBBM, HB-GAM, HBGF-8, HBNF-1, heparin-binding brain mitogen, heparin-binding growth factor 8, heparin-binding neurite outgrowth-promoting factor 1, OSF-1, osteoblast-specific factor 1
Official Symbol: PTN
Accession Number(s): NP_002816.1
Human GeneID(s): 5764
Non-Human GeneID(s): 19242 (mouse), 24924 (rat)

Immunogen

Peptide with sequence C-KEGKKQEKMLD, from the C Terminus of the protein sequence according to NP_002816.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 18kDa band observed in lysates of Mouse fetal Brain (calculated MW of 18.9kDa according to Mouse NP_032999.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Prostate shows distinct staining at the base of the glands. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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