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Goat Anti-PMSCL1 Antibody (EB08993)

Code Name Applications Availability Size Tested species Price Grade  
EB08993 Goat Anti-PMSCL1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08993 1
EB08993 (0.3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EXOSC9, exosome component 9, PM/Scl-75, PMSCL1, RRP45, Rrp45p, p5, p6, P75 polymyositis-scleroderma overlap syndrome associated autoantigen, PMSCL autoantigen, 75kD, autoantigen PM/Scl 1, polymyositis/scleroderma autoantigen 1 (75kD), polymyositis/scleroderma autoantigen 1, 75kDa
Official Symbol: EXOSC9
Accession Number(s): NP_001029366.1; NP_005024.2
Human GeneID(s): 5393
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001029366.1; NP_005024.2).

Immunogen

Peptide with sequence C-RTQTTSAKQEKAP , from the C Terminus of the protein sequence according to NP_001029366.1; NP_005024.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 75kDa band observed in lysates of cell line Jurkat (calculated MW of 50.8kDa according to NP_001029366.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Alderuccio, J Exp Med. 1991 Apr 1;173(4):941-52; PMID: 2007859). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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