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Goat Anti-POLDIP2 Antibody (EB10591)

Related Products: EBP10590 - Immunizing Peptide, EBP10591 - Immunizing Peptide, Goat Anti-POLDIP2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10591 Goat Anti-POLDIP2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10591 1
EB10591 (1µg/ml) staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35µg protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
EB10591 2
EB10590 (0.5µg/ml) staining of Rat VSMC lysate (35µg protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence. Data provided by Everest Grant winner Dr. B. Lassegue, Emory University, Atlanta, USA
EB10591 3
EB10591 (2µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: POLDIP2, polymerase (DNA-directed), delta interacting protein 2, DKFZp586F1524, PDIP38, POLD4, 38 kDa DNA polymerase delta interaction protein, p38, polymerase delta interacting protein 38, polymerase delta-interacting protein 2
Official Symbol: POLDIP2
Accession Number(s): NP_056399.1
Human GeneID(s): 26073
Non-Human GeneID(s): 67811 (mouse)

Immunogen

Peptide with sequence KTHTYYQVLIDARDC, from the internal region of the protein sequence according to NP_056399.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 37kDa band observed in lysates of cell lines K562 and MCF7 and of Mouse Liver (calculated MW of 42.0kDa according to NP_056399.1). Approx 37kDa band observed in Rat Vascular Smooth Muscle Cells lysates. The band dimished upon siRNA knock down. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Rat, Dog, Cow

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