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Goat Anti-polo-like kinase 5 (aa309-322) Antibody (EB12016)

Code Name Applications Availability Size Tested species Price Grade  
EB12016 Goat Anti-polo-like kinase 5 (aa309-322) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB12016 1
EB12016 (2µg/ml) staining of Cerebral Cortex ysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLK5, polo-like kinase 5, PLK-5, PLK5P, SgK384ps, inactive serine/threonine-protein kinase PLK5, polo-like kinase 5, pseudogene
Official Symbol: PLK5
Accession Number(s): NP_001230008.1
Human GeneID(s): 126520

Immunogen

Peptide with sequence SLDVPRSHGCAPTT, from the internal region of the protein sequence according to NP_001230008.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 37kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 36.3kDa according to NP_001230008.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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