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Code Name Applications Availability Size Tested species Price Grade  
EB10018 Goat Anti-POU2AF1 Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10018 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with EB10018(1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with EB10018(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB10018 2
In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5µg/ml.
Click here to download PDF datasheet.

Target Protein

Principal Names: BOB1, OBF1, OBF-1, OCAB, POU class 2 associating factor 1, POU domain, class 2, associating factor 1, POU2AF1
Official Symbol: POU2AF1
Accession Number(s): NP_006226.2
Human GeneID(s): 5450

Immunogen

Peptide with sequence C-DSDAYALNHTLSVE, from the C Terminus of the protein sequence according to NP_006226.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2µg/ml.
IHC: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5µg/ml.

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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