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Goat Anti-POU2F2 / OCT2 Antibody (EB10021)

Code Name Applications Availability Size Tested species Price Grade  
EB10021 Goat Anti-POU2F2 / OCT2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB10021 1
EB10021 (0.1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: homeobox protein, OCT2, Oct-2, OTF2, POU class 2 homeobox 2, POU domain, class 2, transcription factor 2, POU2F2
Official Symbol: POU2F2
Accession Number(s): NP_002689.1
Human GeneID(s): 5452

Immunogen

Peptide with sequence KQGLDSPSEHTDTER-C, from the N Terminus of the protein sequence according to NP_002689.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human Tonsil and Mouse Thymus lysates (calculated MW of 49.5kDa according to NP_002689.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pelis et al, Am J Physiol Renal Physiol. 2006 May;290(5):F1118-26. PMID: 16368738). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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