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Goat Anti-POU3F3 / BRN1 / OCT8 Antibody (EB10027)

Related Products: EBP10027 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10027 Goat Anti-POU3F3 / BRN1 / OCT8 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Mouse $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10027 1
EB10027 (1µg/ml) staining of Mouse Spinal Cord (A) and Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10027 2
EB10027 Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10027 3
EB10027 Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: Brain-1, BRN1, OTF8, POU class 3 homeobox 3, POU domain, class 3, transcription factor 3, POU3F3, OTF-8, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8
Official Symbol: POU3F3
Accession Number(s): NP_006227.1
Human GeneID(s): 5455
Non-Human GeneID(s): 18993 (mouse), 192109 (rat)

Immunogen

Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 48kDa band observed in Mouse Spinal Cord lysates and approx. 50kDa in Mouse Brain lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat

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