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Goat Anti-POU4F3 Antibody (EB10034)

Related Products: EBP10034 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10034 Goat Anti-POU4F3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10034 1
EB10034 (0.2µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: BRN3C, DFNA15, MGC138412, POU class 4 homeobox 3, POU domain, class 4, transcription factor 3, POU4F3
Official Symbol: POU4F3
Accession Number(s): NP_002691.1
Human GeneID(s): 5459
Important Comments: This antibody is expected not to cross-react to the similar POU4F1 and POU4F2.

Immunogen

Peptide with sequence C-EAAYREKNSKPE, from the internal region of the protein sequence according to NP_002691.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 37.1kDa according to Human NP_002691.1). Recommended concentration: 0.2-0.6µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

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