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Goat Anti-PPP1R15A / GADD34 Antibody - Trial (EB05034-T)

Related Products: EBP05034 - Immunizing Peptide, Goat Anti-PPP1R15A / GADD34 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05034-T Goat Anti-PPP1R15A / GADD34 Antibody - Trial Pep-ELISA, WB, IHC, IF, FC Yes 20µg specific antibody in 40µl Human $105.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05034-T 1
EB05034 (0.3µg/ml) staining of HepG2 (A) and negative control KLY (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
EB05034-T 2
EB05034 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05034-T 3
EB05034 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05034-T 4
EB05034 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05034-T 5
EB05034 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A
Official Symbol: PPP1R15A
Accession Number(s): NP_055145.3
Human GeneID(s): 23645

Immunogen

Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.
IHC: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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