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Goat Anti-PPP2CA / PPP2CB Antibody (EB05230)

Code Name Applications Availability Size Tested species Price Grade  
EB05230 Goat Anti-PPP2CA / PPP2CB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05230 1
EB05230 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05230 2
EB05230 (0.03µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05230 3
EB05230 (10µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05230 4
EB05230 (10µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP2CA, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PP2Ac, PP2CA, RP-C, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isoform, replication protein C, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, PP2CB, PP2A-beta, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase type 2A catalytic subunit, serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
Official Symbol: PPP2CA / PPP2CB
Accession Number(s): NP_002706.1; NP_004147.1
Human GeneID(s): 5515 , 5516
Non-Human GeneID(s): 19052 (mouse), 24672 (rat)
Important Comments: Two variants NP_001009552.1 and NP_004147.1 represent identical protein.

Immunogen

Peptide with sequence C-EPHVTRRTPDYFL, from the C Terminus of the protein sequence according to NP_002706.1; NP_004147.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 36kDa band observed in Human Cerebellum and in Mouse, Rat and Pig Brain lysates (calculated MW of 35.6kDa according to Human NP_002706.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney and Thyroid. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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