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Code Name Applications Availability Size Tested species Price Grade  
EB08233 Goat Anti-PPP2R3A Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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EB08233 1
EB08233 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP2R3A, protein phosphatase 2 (formerly 2A), regulatory subunit B\1\1, alpha, PPP2R3, PR130, PR72, PP2A, subunit B, B\1\1-PR72/PR130, PP2A, subunit B, B72/B130 isoforms, PP2A, subunit B, R3 isoform, Serine/threonine protein phosphatase 2A, 72/130 kDa regul
Official Symbol: PPP2R3A
Accession Number(s): NP_002709.2; NP_871626.1
Human GeneID(s): 5523
Important Comments: This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).


Peptide with sequence C-QKDVENDGPEPSD, from the internal region of the protein sequence according to NP_002709.2; NP_871626.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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