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Goat Anti-PPP2R5D Antibody (EB05232)

Code Name Applications Availability Size Tested species Price Grade  
EB05232 Goat Anti-PPP2R5D Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05232 1
EB05232 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PPP2R5D, MGC2134, MGC8949, protein phosphatase 2, regulatory subunit B (B56), delta isoform, PP2A, B subunit, B\1 delta isoform, PP2A, B subunit, R5 delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform, protein phosphatase 2, regulatory subunit B\1, delta isoform, B56D, delta isoform of regulatory subunit B56, protein phosphatase 2A, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Official Symbol: PPP2R5D
Accession Number(s): NP_006236.1; NP_851307.1; NP_851308.1
Human GeneID(s): 5528
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_006236.1; NP_851307.1; NP_851308.1).

Immunogen

Peptide with sequence C-KRAEEFLTASQEAL, from the C Terminus of the protein sequence according to NP_006236.1; NP_851307.1; NP_851308.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:20000.
Western blot: Approx 70kDa and 60kDa bands seen in 293 lysate (Predicted MW of 68kDa and 62kDa according to NP_006236 and AAH01175, respectively). Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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