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Goat Anti-Prefoldin Antibody (EB06290)

Related Products: EBP06290 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06290 Goat Anti-Prefoldin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06290 1
EB06290 (1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PFDN1, PDF, PFD1, prefoldin 1, prefoldin subunit 1
Official Symbol: PFDN1
Accession Number(s): NP_002613.2
Human GeneID(s): 5201
Non-Human GeneID(s): 67199 (mouse)

Immunogen

Peptide with sequence C-DNIREMLMARRAQ, from the C Terminus of the protein sequence according to NP_002613.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 14kDa band observed in lysates of cell line K562 (calculated MW of 14.2kDa according to NP_002613.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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