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Goat Anti-PRKD1 (aa233-246) Antibody (EB12885)

Related Products: EBP12885 - Immunizing Peptide, EBP12886 - Immunizing Peptide, Goat Anti-PRKD1 (aa383-395) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12885 Goat Anti-PRKD1 (aa233-246) Antibody Pep-ELISA, WB-Trf, IP, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12885 1
HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with EB12885 (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.
EB12885 2
HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug EB12885. The precipitate was subsequently probed in Western blot using EB12885 at 1ug/ml. The secondary anti-goat picks up the heavy chain of EB12885 used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA
Click here to download PDF datasheet.

Target Protein

Principal Names: PRKD1, PKC-MU, PKCM, PKD, PRKCM;
Official Symbol: PRKD1
Accession Number(s): NP_002733.2
Human GeneID(s): 5587
Non-Human GeneID(s): 18760 (mouse), 85421 (rat)

Immunogen

Peptide with sequence C-QKSPSESFIGREKR, from the internal region of the protein sequence according to NP_002733.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3ug/ml
IHC: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Döppler et al, Sci Rep. 2016 Sep 21;6:33758 PMID 27649783.

Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

Specific Reference

This antibody has been successfully used in the following paper:
Döppler H, Panayiotou R, Reid EM, Maimo W, Bastea L, Storz P.
The PRKD1 promoter is a target of the Kras-NF-κB pathway in pancreatic cancer.
Sci Rep. 2016 Sep 21;6:33758.
PMID: 27649783

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