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Goat Anti-PRL1 / PTP4A1 Antibody (EB06456)

Related Products: EBP05243 - Immunizing Peptide, EBP06456 - Immunizing Peptide, Goat Anti-PTP4A1 / PRL-1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06456 Goat Anti-PRL1 / PTP4A1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $392.00 Elite

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EB06456 1
EB06456 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06456 2
EB06456 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: PTP4A1, HH72, PRL1, PRL-1, PTPCAAX1, PTP(CAAX1), protein tyrosine phosphatase type IVA, member 1, Protein tyrosine phosphatase IVA1, RP5-1148A21.2, DKFZp779M0721, protein tyrosine phosphatase type IVA protein 1
Official Symbol: PTP4A1
Accession Number(s): NP_003454.1
Human GeneID(s): 7803

Immunogen

Peptide with sequence CFKDSNGHRNN, from the C Terminus of the protein sequence according to NP_003454.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: This antibody has been successfully used in Western Blot as descibed in the following papers, PMID: 18997816 and PMID: 36793871.

Immunofluorescence: Strong expression of the protein seen in HeLa and NIH3T3 cells. Recommended concentration: 10µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Achiwa H et al. (2007) PMID: 17234774.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in WB on Mouse:
Byungtae Hwang, Min-Gi Kwon, Min Ji Cho, Nam-Kyung Lee, Jangwook Lee, Jeong Woong Lee, Kyoung-Jin Oh, Kwang-Hee Bae, Jung Hwan Hwang, Jeong-Ki Min, Jong-Gil Park
Hepatic PTP4A1 ameliorates high-fat diet-induced hepatosteatosis and hyperglycemia by the activation of the CREBH/FGF21 axis.
Theranostics. 2023 Jan 22;13(3):1076-1090.
PMID: 36793871

This antibody has been successfully used in WB on Human:
Min SH, Kim DM, Heo YS, Kim YI, Kim HM, Kim J, Han YM, Kim IC, Yoo OJ.
New p53 target, phosphatase of regenerating liver 1 (PRL-1) downregulates p53.
Oncogene. 2009 Jan 29;28(4):545-54.
PMID: 18997816

This antibody has been successfully used in the following paper:
Achiwa H, Lazo JS.
PRL-1 tyrosine phosphatase regulates c-Src levels, adherence, and invasion in human lung cancer cells.
Cancer Res. 2007 Jan 15;67(2):643-50.
PMID: 17234774

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