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Goat Anti-PRMT5 Antibody (EB09534)

Related Products: EBP09534 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09534 Goat Anti-PRMT5 Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09534 1
EB09534 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09534 2
EB09534 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09534 3
EB09534 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRMT5, protein arginine methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog
Official Symbol: PRMT5
Accession Number(s): NP_006100.2; NP_001034708.1; NP_001269882.1; NP_001269883.1; NP_001269884.1; NP_001269885.1
Human GeneID(s): 10419
Non-Human GeneID(s): 27374 (mouse), 364382 (rat)
Important Comments: This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1).

Immunogen

Peptide with sequence RACREKDRDPEAQ, from the internal region of the protein sequence according to NP_006100.2; NP_001034708.1; NP_001269882.1; NP_001269883.1; NP_001269884.1; NP_001269885.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary testing showed a band at approx. 70kDa in A431 and HepG2 nuclear cell lysate after 1-2ug/ml antibody staining. (calculated MW of 72.7kDa according to NP_006100.2). Primary incubation 1 hour at room temperature

Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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