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Goat Anti-Progranulin aa248-259 / Granulin-3 Antibody (EB11324)

Related Products: EBP08934 - Immunizing Peptide, EBP11324 - Immunizing Peptide, Goat Anti-Granulin / GRN Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11324 Goat Anti-Progranulin aa248-259 / Granulin-3 Antibody Pept-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11324 1
EB11324 (1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: acrogranin, GEP, GP88, granulin, granulin-epithelin, granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, proepithelin, progranulin, GRN
Official Symbol: GRN
Accession Number(s): NP_002078.1
Human GeneID(s): 2896
Important Comments: This antibody is expected to recognize the C-Terminal part of Granulin-3.

Immunogen

Peptide with sequence PQDTVCDLIQSK, from the internal of the protein sequence according to NP_002078.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 75kDa band observed in lysates of cell lines A431, A549 and HeLa (calculated MW of 63.5kDa according to NP_002078.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (He and Bateman, Cancer Res. 1999 Jul 1;59(13):3222-9. PMID: 10397269). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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