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Code Name Applications Availability Size Tested species Price Grade  
EB11821 Goat Anti-PROM1 / CD133 Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $324.00 Elite

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EB11821 1
EB11821 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmicstaining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: AC133, antigen AC133, CD133, CORD12, hematopoietic stem cell antigen, hProminin, MCDR2, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, prominin 1, prominin-1, prominin-like 1, prominin-like protein 1, PROML1, RP41, STGD4
Official Symbol: PROM1
Accession Number(s): NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1
Human GeneID(s): 8842
Important Comments: This antibody is expected to recognize all reported isoforms (NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1). Reported variants represent identical protein: NP_001139320.1, NP_001139319.1


Peptide with sequence RHATPTTRGCVSN, from the internal region of the protein sequence according to NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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