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Goat Anti-PSMA4 Antibody (EB10277)

Related Products: EBP10277 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10277 Goat Anti-PSMA4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10277 1
EB010277 (1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10277 2
EB010277 (0.3µg/ml) staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HC9, HsT17706, macropain subunit C9, MGC111191, MGC12467, MGC24813, multicatalytic endopeptidase complex subunit C9, proteasome (prosome, macropain) subunit, alpha type, 4, proteasome alpha 4 subunit, proteasome component C9, proteasome subun, PSC9, PSMA4
Official Symbol: PSMA4
Accession Number(s): NP_002780.1; NP_001096138.1
Human GeneID(s): 5685
Non-Human GeneID(s): 26441 (mouse), 29671 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1

Immunogen

Peptide with sequence C-SDANVLTNELR, from the internal region of the protein sequence according to NP_002780.1; NP_001096138.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 28kDa band observed in lysates of cell lines K562, NIH3T3 and in lysates of Human Tonsil, Human Peripheral Blood Mononucleocytes and in Mouse and Rat Spleen (calculated MW of 29.5kDa according to NP_002780.1). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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