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Code Name Applications Availability Size Tested species Price Grade  
EB10295 Goat Anti-PSMB4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10295 1
EB10295 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10295 2
EB10295 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HN3, HsN3, macropain beta chain, multicatalytic endopeptidase complex beta chain, PROS26, proteasome (prosome, macropain) subunit, beta type, 4, proteasome beta 4 subunit, proteasome beta chain, proteasome chain 3, proteasome subunit HsN3, PSMB4
Official Symbol: PSMB4
Accession Number(s): NP_002787.2
Human GeneID(s): 5692
Non-Human GeneID(s): 19172 (mouse), 58854 (rat)

Immunogen

Peptide with sequence C-QIATVTEKGVE, from the internal region of the protein sequence according to NP_002787.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 25kDa band observed in lysates of cell lines NIH3T3, HEK293, HeLa, Jurkat and K562 (calculated MW of the mature protein 24.3kDa according to aa46-264 of NP_002787.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skin. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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