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Goat Anti-PSMB7 Antibody (EB10298)

Code Name Applications Availability Size Tested species Price Grade  
EB10298 Goat Anti-PSMB7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $423.00 Elite

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EB10298 1
EB10298 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Target Protein

Principal Names: macropain chain Z, multicatalytic endopeptidase complex chain Z, proteasome (prosome, macropain) subunit, beta type, 7, proteasome beta 7 subunit, proteasome catalytic subunit 2, proteasome subunit alpha, proteasome subunit Z, PSMB7
Official Symbol: PSMB7
Accession Number(s): NP_002790.1
Human GeneID(s): 5695
Non-Human GeneID(s): 19177 (mouse), 85492 (rat)

Immunogen

Peptide with sequence C-RNAVLEADFAKR, from the internal region of the protein sequence according to NP_002790.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in lysates of cell lines HEK 293, HeLa and Jurkat and of Mouse Fetal Brain (calculated MW of 30.0kDa according to NP_002790.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig