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Goat Anti-PTBP1 / PTB Antibody (EB06088)

Related Products: EBP06088 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06088 Goat Anti-PTBP1 / PTB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06088 1
EB06088 (0.003µg/ml) staining of K562 (A), nuclear Jurkat (B), KNRK (C) and (0.001ug/ml) NIH3T3 (D) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06088 2
EB06088 (0.01µg/ml) staining of Mouse Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06088 3
EB06088 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06088 4
EB06088 Negative Control showing staining of paraffin embedded Human Kidney , with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), HNRNP-I, MGC10830, MGC8461, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I
Official Symbol: PTBP1
Accession Number(s): NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1
Non-Human GeneID(s): 19205 (mouse), 29497 (rat)
Important Comments: This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).

Immunogen

Peptide with sequence DGIVPDIAVGTKR-C, from the N Terminus of the protein sequence according to NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Mouse Ovary lysates and in lysates of cell line NIH3T3, K562, KNRK and Nuclear Jurkat (calculated MW of 57.2kDa according to Human NP_114368.1 and 56.9kDa according to Mouse NP_032982.2 and Rat NP_071961.2). Recommended concentration: 0.001-0.01µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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