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Goat Anti-PTEN Antibody (EB06544)

Related Products: EBP06544 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06544 Goat Anti-PTEN Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB06544 1
EB06544 (1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTEN, BZS, MHAM, TEP1, MMAC1, PTEN1, MGC11227, phosphatase and tensin homolog (mutated in multiple advanced cancers 1), tensin homolog, mutated in multiple advanced cancers 1, MMAC1 phosphatase and tension homolog deleted on chromosome 10
Official Symbol: PTEN
Accession Number(s): NP_000305.3
Human GeneID(s): 5728

Immunogen

Peptide with sequence CNEPFDEDQHTQIT, from the internal region (near the C Terminus) of the protein sequence according to NP_000305.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in lysates of cell line A431 and 50kDa in lysates of cell line NIH3T3 (calculated MW of 47.1kDa according to Human NP_000305 and Mouse NP_032986.1). Recommended concentration: 1-3µg/ml.

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from a customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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