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Code Name Applications Availability Size Tested species Price Grade  
EB08205 Goat Anti-PTGER3 / EP3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08205 1
EB08205 (1µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTGER3, EP3, prostaglandin E receptor 3 (subtype EP3), EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, MGC141828, MGC141829, MGC27302, PGE receptor, EP3 subtype, prostaglandin E receptor 3, subtype EP3, prostaglandin E2 receptor, prostaglandin receptor (PGE-2), pr
Official Symbol: PTGER3
Accession Number(s): NP_000948.2; NP_942005.2; NP_942006.1
Human GeneID(s): 5733
Important Comments: This antibody is expected to recognize isoforms 1, 2 and 3 (NP_000948.2, NP_942005.2 and NP_942006.1).

Immunogen

Peptide with sequence KLLREPCSVQLS, from the C Terminus of the protein sequence according to NP_000948.2; NP_942005.2; NP_942006.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 55kDa band observed in Human Adipose, Kidney, Pancreas and Placenta lysates (calculated MW of 47.3kDa according to NP_000948.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sung et al, , Cancer Res. 2005 Oct 15;65(20):9304-11; PMID: 16230392). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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