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Goat Anti-RANBP8 / IPO8 Antibody (EB05535)

Related Products: EBP05535 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05535 Goat Anti-RANBP8 / IPO8 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB05535 1
EB05535 (4µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
Click here to download PDF datasheet.

Target Protein

Principal Names: IPO8, importin 8, RANBP8, RAN-binding protein 8, FLJ26580, RAN binding protein 8
Official Symbol: IPO8
Accession Number(s): NP_006381.2; NP_001177924.1
Human GeneID(s): 10526
Important Comments: This antibody is expected to recognise isoform 1 (NP_006381.2) and isoform 2 (NP_001177924.1).

Immunogen

Peptide with sequence C-SAFNFGTVPSNN, from the C Terminus of the protein sequence according to NP_006381.2; NP_001177924.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

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