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Goat Anti-RANBPM / RANBP9 Antibody (EB06028)

Related Products: EBP06028 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06028 Goat Anti-RANBPM / RANBP9 Antibody Pep-ELISA, IHC, ICC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06028 1
EB06028 (3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer Ph9, HRP-staining.
EB06028 2
EB06028 (10µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RANBP9, RanBPM, RAN binding protein 9, RANBPM, novel centrosomal protein RanBPM, ran binding protein, centrosomal
Official Symbol: RANBP9
Accession Number(s): NP_005484.2
Human GeneID(s): 10048
Non-Human GeneID(s): 56705 (mouse)
Important Comments: This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1).

Immunogen

Peptide with sequence GSCAFATVEDYLH, from the C Terminus of the protein sequence according to NP_005484.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1µg/ml.
IHC: In paraffin embedded Human Tonsil stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration: 3µg/ml. Paraffin embedded Human Testis. Recommended concentration: 10µg/ml.

Immunocytochemistry: A customer reported successful use on HeLa.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Pig

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