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Goat Anti-RARA Antibody (EB06672)

Related Products: EBP06672 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06672 Goat Anti-RARA Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06672 1
EB06672 (1µg/ml) staining of Human Breast (A) and Breast cancer (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: RARA, RAR, NR1B1, retinoic acid receptor, alpha, Retinoic acid receptor, alpha polypeptide, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form
Official Symbol: RARA
Accession Number(s): NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1
Human GeneID(s): 5914
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.

Immunogen

Peptide with sequence C-SPSLSPSSHRSSPATQSP, from the C Terminus of the protein sequence according to NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 55kDa band observed in Human Breast and Breast cancer lysates (calculated MW of 50.8kDa according to NP_000955.1and NP_001019980.1). Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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