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Goat Anti-RASIP1 / RAIN Antibody (EB06422)

Related Products: EBP06422 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06422 Goat Anti-RASIP1 / RAIN Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06422 1
EB06422 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: Ras-interacting protein 1, RAIN, FLJ20401, RASIP1, Ras interacting protein 1
Official Symbol: RASIP1
Accession Number(s): NP_060275.2
Human GeneID(s): 54922

Immunogen

Peptide with sequence CANYRHGPPVATSP, from the C Terminus of the protein sequence according to NP_060275.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Experiments gave bands at approx 95kDa, 75kDa and 45kDa in A549 Lung Carcinoma lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the predicted size of 110kDa according to NP_060275. The detected bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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