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Goat Anti-RASSF2 Antibody (EB08511)

Code Name Applications Availability Size Tested species Price Grade  
EB08511 Goat Anti-RASSF2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB08511 1
EB08511 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08511 2
EB08511 (3.75µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08511 3
EB08511 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RASSF2, Ras association (RalGDS/AF-6) domain family 2, DKFZp781O1747N: KIAA0168, Ras association domain family 2, rasfadin
Official Symbol: RASSF2
Accession Number(s): NP_055552.1; NP_739580.1
Human GeneID(s): 9770
Important Comments: Reported variants represent identical protein: NP_055552.1; NP_739580.1.

Immunogen

Peptide with sequence PCGQDKYISKNE, from the N Terminus of the protein sequence according to NP_055552.1; NP_739580.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Spleen, Thymus and Liver. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

Specific Reference

This antibody has been successfully used in IHC and IF in Human:
Perez-Janices N, Blanco-Luquin I, Torrea N, Liechtenstein T, Escors D, Cordoba A, Vicente-Garcia F, Jauregui I, De La Cruz S, Illarramendi JJ, Coca V, Berdasco M, Kochan G, Ibañez B, Lera JM, Guerrero-Setas D.
Differential involvement of RASSF2 hypermethylation in breast cancer subtypes and their prognosis.
Oncotarget. 2015 Sep 15;6(27):23944-58.
PMID: 26284587

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