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Goat Anti-RDH5 (aa 19-31) Antibody (EB10078)

Related Products: EBP10077 - Immunizing Peptide, EBP10078 - Immunizing Peptide, Goat Anti-RDH5 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10078 Goat Anti-RDH5 (aa 19-31) Antibody Pep-ELISA, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10078 1
EB10078 (5µg/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB10078 2
EB10078 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong Endoplasmic Reticulum and weak cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10078 3
EB10078 Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: 11-cis RDH, 9-cis-retinol specific dehydrogenase, FLJ39337, FLJ97089, HSD17B9, RDH1, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), retinol dehydrogenase 5 (11-cis/9-cis), SDR9C5, short chain dehydrogenase/reductase family 9C,, RDH5
Official Symbol: RDH5
Accession Number(s): NP_002896.2
Human GeneID(s): 5959
Non-Human GeneID(s): 19682 (mouse)

Immunogen

Peptide with sequence C-RDRQSLPASNAFV, from the internal region of the protein sequence according to NP_002896.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary testing showed a band at approx..32kDa in Human Heart and approx. 35kDa in Human Breast lysate, after 0.3ug/ml antibody staining. (calculated MW of 35.0kDa according to NP_002896.2). Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration: 5-10µg/ml.

Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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