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Goat Anti-RGS5 Antibody (EB05422)

Code Name Applications Availability Size Tested species Price Grade  
EB05422 Goat Anti-RGS5 Antibody Allow up to 10 days for delivery 100µg specific antibody in 200µl Human $255.00 Aspiring

Ordering Information for United States of America (change)

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Target Protein

Principal Names: RGS5, regulator of G-protein signalling 5, Regulator of G protein signaling-5, RP11-430G6.2, MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129
Official Symbol: RGS5
Accession Number(s): NP_003608.1
Human GeneID(s): 8490
Important Comments: This antibody is expected to recognise isoform 1 (NP_003608.1) only.

Immunogen

Peptide with sequence KGLAALPHSCLER, from the N Terminus of the protein sequence according to NP_003608.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Western blot: Approx 40kDa band seen in Human Lung lysate. Recommended for use at 1-3µg/ml. Please note that we cannot find any reference indicating what size RGS5 runs on SDS PAGE; we also cannot find any obvious reason for it to run at 40kDa. Do you know of anyone who has made an antibody and found it running at the expected weight - it would be very useful for us to know for the purpose of further testing.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow