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Goat Anti-Rhophilin 2 (Internal) Antibody (EB06853)

Related Products: EBP06248 - Immunizing Peptide, EBP06853 - Immunizing Peptide, Goat Anti-Rhophilin 2 (N Terminus) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06853 Goat Anti-Rhophilin 2 (Internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06853 1
EB06853 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein
Official Symbol: RHPN2
Accession Number(s): NP_149094.3
Human GeneID(s): 85415

Immunogen

Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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