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Goat Anti-RNF39 / LIRF Antibody (EB05820)

Related Products: EBP05820 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05820 Goat Anti-RNF39 / LIRF Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05820 1
EB05820 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05820 2
EB05820 (10µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF39, LIRF, ring finger protein 39, HZFW1, HZFw1 protein, LTP (long-term potentiation) induced RING finger protein, DADB-60O11.8, HZF, HZFW
Official Symbol: RNF39
Accession Number(s): NP_079512.1; NP_739575.1
Human GeneID(s): 80352
Non-Human GeneID(s): 171387 (rat)
Important Comments: This antibody is expected to recognize 2 of the 3 reported isoforms (NP_079512.1 and NP_739575.1 but not NP_739576.1)

Immunogen

Peptide with sequence CDPRAPLRIVPAES, from the C Terminus of the protein sequence according to NP_079512.1; NP_739575.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in Human Brain lysates (predicted MWs of 41kDa and 48kDa according to NP_079512.1 and NP_739575.1). Recommended for use at 1-3µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Rat, Pig

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