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Code Name Applications Availability Size Tested species Price Grade  
EB09769 Goat Anti-RNF7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09769 1
EB09769 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09769 2
EB09769 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF7, ring finger protein 7, CKBBP1, ROC2, SAG, OTTHUMP00000180645, regulator of cullins 2, sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, zinc RING finger protein SAG
Official Symbol: RNF7
Accession Number(s): NP_055060.1
Human GeneID(s): 9616
Non-Human GeneID(s): 19823 (mouse)
Important Comments: This antibody is expected to recognize isoform 1 (NP_055060.1).

Immunogen

Peptide with sequence DACLRCQAENKQE, from the internal region of the protein sequence according to NP_055060.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3µg/ml. An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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