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Goat Anti-ROBO1 / DUTT1 (Internal) Antibody (EB07058)

Related Products: EBP07058 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07058 Goat Anti-ROBO1 / DUTT1 (Internal) Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07058 1
EB07058 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Plasma membrane and Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07058 2
EB07058 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Plasma membrane and ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07058 3
EB07058 (5µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07058 4
EB07058 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
EB07058 5
EB07058 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07058 6
EB07058 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila), HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
Official Symbol: ROBO1
Accession Number(s): NP_002932.1; NP_598334.1
Human GeneID(s): 6091
Non-Human GeneID(s): 19876 (mouse), 58946 (rat)
Important Comments: Not expected to cross-react with ROBO2 or ROBO3.

Immunogen

Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Brain (Cortex) and Kidney. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the Plasma membrane and Golgi/ER of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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