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Goat Anti-SAE2 / UBA2 Antibody (EB06098)

Code Name Applications Availability Size Tested species Price Grade  
EB06098 Goat Anti-SAE2 / UBA2 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06098 1
EB06098 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06098 2
EB06098 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and weak cytoplasmic weak staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: UBA2, SAE2, SUMO-1 activating enzyme subunit 2
Official Symbol: SAE2
Accession Number(s): NP_005490
Human GeneID(s): 10054
Non-Human GeneID(s): 50995 (mouse)

Immunogen

Peptide with sequence ALSRGLPRELAEA-C, from the N Terminus of the protein sequence according to NP_005490.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013;77(7):1575-8. PMID: 23832333.

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Kanemaru A, Saitoh H.
High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system.
Biosci Biotechnol Biochem. 2013;77(7):1575-8.
PMID: 23832333

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