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Goat Anti-SALL1 (aa29-43) Antibody (EB09625)

Related Products: EBP07779 - Immunizing Peptide, EBP09625 - Immunizing Peptide, Goat Anti-SALL1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09625 Goat Anti-SALL1 (aa29-43) Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB09625 1
EB09625 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09625 2
EB09625 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SALL1, sal-like 1 (Drosophila), HSAL1, TBS, ZNF794, sal-like 1, spalt-like transcription factor 1
Official Symbol: SALL1
Accession Number(s): NP_002959.2
Human GeneID(s): 6299
Non-Human GeneID(s): 58198 (mouse)

Immunogen

Peptide with sequence EKGQPSRPTKSKDAH-C, from the N Terminus of the protein sequence according to NP_002959.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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