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Goat Anti-SCAP2 / PRAP Antibody (EB05356)

Code Name Applications Availability Size Tested species Price Grade  
EB05356 Goat Anti-SCAP2 / PRAP Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $392.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]

EB05356 1
HEK293 overexpressing SKAP2 (RC206119) and probed with EB05356 (mock transfection in first lane), tested by Origene.
EB05356 2
EB05356 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SKAP2, SCAP2, PRAP, src family associated phosphoprotein 2, MGC10411, MGC33304, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, src family associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adaptor protein
Official Symbol: SKAP2
Accession Number(s): NP_003921.2
Human GeneID(s): 8935


Peptide with sequence C-GLVPKAYIMEMYDI, from the C Terminus of the protein sequence according to NP_003921.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2µg/ml. An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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