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Code Name Applications Availability Size Tested species Price Grade  
EB12300 Goat Anti-SCARB1 / SR-BI Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB12300 1
EB12300 (0.01µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12300 2
EB12300 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SCARB1, scavenger receptor class B, member 1, CD36L1, CLA-1, CLA1, HDLQTL6, SR-BI, SRB1, CD36 and LIMPII analogous 1, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1, scavenger receptor class B member 1, scavenger receptor class B type III
Official Symbol: SCARB1
Accession Number(s): NP_005496.4
Human GeneID(s): 949
Important Comments: This antibody is expected to recognize isoform 1 (NP_005496.4) only.

Immunogen

Peptide with sequence C-TSAPKGSVLQEAK, from the C Terminus of the protein sequence according to NP_005496.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 85kDa band observed in Human Adrenal gland lysates (calculated MW of 57.0kDa according to NP_005496.4). We do note that SCARB1 is a glycoprotein which makes a higher size band to be expected, and this is consistent with observations with different antibodies from other sources. Recommended concentration: 0.01-0.03µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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