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Goat Anti-SCRIB / Scribble Antibody (EB09387)

Code Name Applications Availability Size Tested species Price Grade  
EB09387 Goat Anti-SCRIB / Scribble Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
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EB09387 1
EB09387 (2µg/ml) staining of HeLa (A), HepG2 (B) and U2OS (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09387 2
EB09387 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble
Official Symbol: SCRIB
Accession Number(s): NP_874365.3; NP_056171.3
Human GeneID(s): 23513
Important Comments: This antibody is expected to recognize both reported isoforms (NP_874365.3; NP_056171.3).

Immunogen

Peptide with sequence C-PEGPGKEKELPGQ, from the internal region of the protein sequence according to NP_874365.3; NP_056171.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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