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Goat Anti-SEPT2 Antibody (EB08931)

Code Name Applications Availability Size Tested species Price Grade  
EB08931 Goat Anti-SEPT2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB08931 1
EB08931 (0.05µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08931 2
EB08931 (2.5µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08931 3
EB08931 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08931 4
EB08931 (2.5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEPT2, septin 2, DIFF6, KIAA0158, NEDD5, Pnutl3, hNedd5, neural precursor cell expressed, developmentally down-regulated 5
Official Symbol: SEPTIN2
Accession Number(s): NP_001008491.1; NP_001269901.1; NP_001269902.1; NP_001307958.1; NP_001336231.1
Human GeneID(s): 4735
Non-Human GeneID(s): 18000 (mouse), 117515 (rat)
Important Comments: This antibody is expected to recognise::NP_001008491.1 (iso a); NP_001269901.1 (iso b); NP_001269902.1 (iso c); NP_001307958.1; (iso d) NP_001336231.1 (iso f).

Immunogen

Peptide with sequence SKQQPTQFINPET-C, from the N terminus of the protein sequence according to NP_001008491.1; NP_001269901.1; NP_001269902.1; NP_001307958.1; NP_001336231.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5kDa according to NP_001008491.1). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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