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Goat Anti-SERCA2 / ATP2A2 Antibody (EB11450)

Code Name Applications Availability Size Tested species Price Grade  
EB11450 Goat Anti-SERCA2 / ATP2A2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
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EB11450 1
EB11450 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11450 2
EB11450 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP2B, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, DAR, DD, DKFZp686P0211, endoplasmic reticulum class 1/2 Ca(2+) ATPase, FLJ20293, FLJ38063, MGC45367, sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, ATP2A2
Official Symbol: ATP2A2
Accession Number(s): NP_001672.1; NP_733765.1
Human GeneID(s): 488
Non-Human GeneID(s): 11938 (mouse), 29693 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).


Peptide with sequence DELNPSAQRDACLN, from the internal region of the protein sequence according to NP_001672.1; NP_733765.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kDa according to NP_001672.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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