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Goat Anti-SERPINB1 (aa130-143) Antibody (EB12397)

Related Products: EBP12396 - Immunizing Peptide, EBP12397 - Immunizing Peptide, Goat Anti-SERPINB1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12397 Goat Anti-SERPINB1 (aa130-143) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12397 1
EB12397 (0.5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
EB12397 2
EB12397 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SERPINB1, serpin peptidase inhibitor, clade B (ovalbumin), member 1, EI, ELANH2, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) prot
Official Symbol: SERPINB1
Accession Number(s): NP_109591.1
Human GeneID(s): 1992

Immunogen

Peptide with sequence C-KTINQWVKGQTEGK, from the internal region of the protein sequence according to NP_109591.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 42-45kDa bands observed in Human Placenta lysates (calculated MW of 42.7kDa according to NP_109591.1). The fainter 45kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide. Recommended concentration: 0.5-2µg/ml.
IHC: Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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