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Goat Anti-SFRP4 Antibody (EB09115)

Related Products: EBP09115 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09115 Goat Anti-SFRP4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09115 1
EB09115 (0.5µg/ml) staining of Human Uterus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09115 2
EB09115 (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SFRP4, secreted frizzled-related protein 4, FRP-4, FRPHE, MGC26498, secreted frizzled-related protein 4
Official Symbol: SFRP4
Accession Number(s): NP_003005.2
Human GeneID(s): 6424
Non-Human GeneID(s): 20379 (mouse), 89803 (rat)

Immunogen

Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence according to NP_003005.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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