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Goat Anti-SGLT1 Antibody (EB09310)

Code Name Applications Availability Size Tested species Price Grade  
EB09310 Goat Anti-SGLT1 Antibody Pep-ELISA, IHC Allow up to 10 days for delivery 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB09310 1
EB09310 (5µg/ml) staining of paraffin embedded Human Small Intestine. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09310 2
EB09310 Negative Control showing staining of paraffin embedded Human Small Intestine, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC5A1, solute carrier family 5 (sodium/glucose cotransporter), member 1, D22S675, NAGT, SGLT1, Human Na+/glucose cotransporter 1 mRNA, complete cds, sodium/glucose cotransporter 1, solute carrier family 5 (sodium/glucose transporter), member 1
Official Symbol: SLC5A1
Accession Number(s): NP_000334.1
Human GeneID(s): 6523

Immunogen

Peptide with sequence C-KETIEIETQVPEKKK, from the internal region of the protein sequence according to NP_000334.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody (previous batch) has been successfully used in Western blot and IHC on Mouse:
Barun Das, Kevin Okamoto, John Rabalais, Jocelyn A. Young, Kim E. Barrett, and Mamata Sivagnanam
Aberrant Epithelial Differentiation Contributes to Pathogenesis in a Murine Model of Congenital Tufting Enteropathy.
Cell Mol Gastroenterol Hepatol. 2021; 12(4): 1353-1371.
PMID: 34198013

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Yui Yamazaki, Kyoko Arita, Shinichi Harada, Shogo Tokuyama
Activation of c-Jun N-terminal kinase and p38 after cerebral ischemia upregulates cerebral sodium-glucose transporter type 1
Journal of Pharmacological Sciences (November 2018)
PMID: 30503674

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Yamazaki Y, Harada S, Wada T, Hagiwara T, Yoshida S, Tokuyama S
Sodium influx through cerebral sodium-glucose transporter type 1 exacerbates the development of cerebral ischemic neuronal damage.
Eur J Pharmacol. 2017 Mar 15;799:103-110.
PMID: 28174043

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