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Goat Anti-Sgta (mouse aa84-97) Antibody (EB11841)

Related Products: EBP11841 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11841 Goat Anti-Sgta (mouse aa84-97) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11841 1
EB11841 (1ug/ml) staining of PD19 (A) and (2µg/ml) K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11841 2
EB11841 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 5330427H01Rik, AI194281, alpha-SGT, D10Ertd190e, Sgt, Sgta, small glutamine-rich tetratricopeptide repeat (TPR) containing protein, small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha, small glutamine-rich tetratricopeptide repeat-containing protein alpha, Stg
Official Symbol: Sgta
Accession Number(s): NP_078775.1
Human GeneID(s): 6449
Non-Human GeneID(s): 52551 (mouse), 64667 (rat)

Immunogen

Peptide with sequence C-PSEEDSAEAERLKT, from the internal region of the protein sequence according to NP_078775.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35kDa band observed in lysates of cell line PD19 and doublet of 30+35kDa in lysates of cell line K562 (calculated MW of 34.3kDa according to NP_078775.1). Recommended concentration: 1-2µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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