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Goat Anti-SHC1; SHC3 Antibody (EB05100)

Related Products: EBP05100 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05100 Goat Anti-SHC1; SHC3 Antibody Contact sales for delivery estimate 100µg specific antibody in 200µl Human $423.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: SHC1, SHC (Src homology 2 domain containing) transforming protein 1, SHC, SHCA, SHC (Src homology 2 domain-containing) transforming protein 1
Official Symbol: SHC1
Accession Number(s): NP_003020; NP_058544
Human GeneID(s): 6464 , 53358
Important Comments: This product is expected to recognize the products of 2 different genes.

Immunogen

Peptide with sequence SELCLQQPVERKQ, from the C Terminus of the protein sequence according to NP_003020; NP_058544.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Western blot: Western blot: Approx 65-70kDa, 45kDa and 30kDa bands seen in U937 lysate. Recommended for use at 1-3 µg/ml.

Please note that the approx. 65-70kDa and 45kD bands correspond to two of the isoforms of SHC, as observed by Pelicci et al., [Cell 1992 Jul 10;70(1):93-104]. We also observed approx. 30-35kDa band. This lower MW band may be recognising an isoform with the accession number AAH33925. Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated the possibility of three isoforms for SHC1.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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