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Goat Anti-SIRT4 Antibody (EB06288)

Code Name Applications Availability Size Tested species Price Grade  
EB06288 Goat Anti-SIRT4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06288 1
EB06288 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06288 2
EB06288 (2.5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIRT4, SIR2L4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae), MGC130046, MGC130047, MGC57437, SIR2L4, sirtuin 4, sir2-like 4, sirtuin 4, sirtuin type 4
Official Symbol: SIRT4
Accession Number(s): NP_036372.1
Human GeneID(s): 23409
Non-Human GeneID(s): 75387 (mouse)

Immunogen

Peptide with sequence SRCGELLPLIDPC, from the C Terminus of the protein sequence according to NP_036372.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38kDa band observed in Human Kidney lysates (calculated MW of 35.2kDa according to NP_036372.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration: 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody has been successfully used in Western blot on Rat:
Thaís Furtado Camargo, Ariane Maria Zanesco, Kayo Augusto Salandin Pacher, Thiago Antonio Moretti Andrade, Armindo Antonio Alves , Maria Esméria Corezola do Amaral
Physiological profile regulation during weight gain and loss by ovariectomized 2 females: importance of SIRT1 and SIRT4
Am J Physiol Endocrinol Metab. 2020 Aug 31. doi: 10.1152/ajpendo.00465.2019
PMID: 32865007

This antibody (previous batch) has been successfully used in Western blot on Dog:
Marfè G, Tafani M, Fiorito F, Pagnini U, Iovane G, De Martino L.
Involvement of FOXO transcription factors, TRAIL-FasL/Fas, and sirtuin proteins family in canine coronavirus type II-induced apoptosis.
PLoS One. 2011;6(11).
PMID: 22087287

This antibody has been successfully used on Rat in the following paper:
do Amaral ME, Ueno M, Oliveira CA, Borsonello NC, Vanzela EC, Ribeiro RA, Alves PL, Barbosa HC, Carneiro EM, Boschero AC.
Reduced expression of SIRT1 is associated with diminished glucose-induced insulin secretion in islets from calorie-restricted rats.
J Nutr Biochem. 2011 Jun;22(6):554-9.
PMID: 20801633

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