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Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody (EB10826)

Related Products: EBP07400 - Immunizing Peptide, EBP10826 - Immunizing Peptide, Goat Anti-SIRT1 Antibody, Goat Anti-SIRT1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB10826 Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10826 1
EB10826 (0.05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10826 2
EB10826 (3.8µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, sir2-like 1, sirtuin type 1
Official Symbol: SIRT1
Accession Number(s): NP_036370.2; NP_001135970.1
Human GeneID(s): 23411
Non-Human GeneID(s): 93759 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).

Immunogen

Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2µg/ml.
IHC: In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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